rat anti ctip2 primary antibody (Danaher Inc)
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Rat Anti Ctip2 Primary Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+ctip2+primary+antibody/pmc11336167-237-17-22
Average 86 stars, based on 1 article reviews
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1) Product Images from "A population of Insula neurons encodes for social preference only after acute social isolation in mice"
Article Title: A population of Insula neurons encodes for social preference only after acute social isolation in mice
Journal: Nature Communications
doi: 10.1038/s41467-024-51389-4
Figure Legend Snippet: a Immunofluorescence confocal images showing Insula Ins neurons (tomato labeling), insula Satb2 staining (yellow labeling), insula Ctip2 labeling (green labeling), and the overlay at low (top, scale 100 µm) and high magnification (bottom, scale 25 µm). At the bottom, white arrows show examples of Tomato and Satb2 colocalizations. b Quantification of Tomato, Satb2, and Ctip2 colocalization in the Insula. c , g Experimental design. d Representative traces showing a collision test and a high-frequency stimulation protocol for an Insula interhemispheric neuron projecting to the other Insula. e Histogram of the onset latency of Insula antidromic responses. f Representative epifluorescent image at low (left, scale bar 500 µm) and high magnification (right, 150 µm) of MBP staining (gray labeling) and Insula Ins neurons (tomato labeling, N = 3 mice). h , i Representative image obtained with electron microscopy showing immunogold GFP labeling of unmyelinated Insula Ins axons passing through the corpus callosum ( h ) or the anterior commissure ( i ) (green arrow, N = 4 mice). The white arrow shows an example of a myelinated axon (scale 500 nm). Rec recording, MBP myelin basic protein, n number of neurons, N number of mice. Data are presented as mean values ± SEM.
Techniques Used: Immunofluorescence, Labeling, Staining, Electron Microscopy
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Incubation:Article Title: A population of Insula neurons encodes for social preference only after acute social isolation in mice Article Snippet: .. Sections were incubated (overnight per 4 °C) with a mouse anti-Satb2 primary antibody (1/300; abcam ab51502), a other:Article Title: A population of Insula neurons encodes for social preference only after acute social isolation in mice. Article Snippet: Afterwashing sectionswere incubatedovernight or 72 h for the CTb experiment at 4 °C with a donkey anti-mouse secondary antibody (labeling of Satb2, 1/500, life technologies A31571, Alexa 647), a donkey anti-rat secondary antibody (labeling of Ctip2 or labeling of MBP, 1/500, life technologies A21209, Alexa 488), a goat anti-mouse secondary antibody (labeling of GAD67,1/500, Invitrogen A21202, Alexa 488), a goat anti-guinea pig secondary antibody (labeling of PV or Neun/Fox3,1/500, Invitrogen A11073, Alexa 488), a donkey anti-goat 647 (labeling of CTb, 1/500, Jackson Immunoresearch, 705- 605-147) a donkey anti-rabbit (labeling GFP,1/500, life technologies A21206, Alexa 488), streptavidin (labeling of biocytin, R&D system NL 999, 1/500, alexa 557). |